Rv2987c 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2987c leuD 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) CDS 3344033 3344629 - 597 198 FALSE

Rv2987c (3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)) is predicted to be co-regulated in modules bicluster_0286 with residual 0.44 and bicluster_0344 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4.30 and 5.80 for bicluster_0286 and 0.10 and 8.00 for bicluster_0344 respectively.

These modules are enriched for following go terms: branched-chain amino acid biosynthetic p..., branched-chain amino acid metabolic proc..., cytosol, cytosolic part .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
3-isopropylmalate dehydratase small subunit isopropylmalate isomerase small subunit
Operon # Operon
1950 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-isopropylmalate dehydratase Valine, leucine and isoleucine biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

C5-Branched dibasic acid metabolism

9
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610124 NP_217503.1 Run
GO:0003861

3-isopropylmalate dehydratase activity

3-isopropylmalate dehydratase activity

Details: 
Catalysis of the reaction: 3-isopropylmalate = 2-isopropylmaleate + H2O.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009098

leucine biosynthetic process

leucine biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: