Rv2995c 3-isopropylmalate dehydrogenase (EC 1.1.1.85)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2995c leuB 3-isopropylmalate dehydrogenase (EC 1.1.1.85) CDS 3352458 3353468 - 1 011 336 FALSE

Rv2995c (3-isopropylmalate dehydrogenase (EC 1.1.1.85)) is predicted to be co-regulated in modules bicluster_0006 with residual 0.61 and bicluster_0515 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 8.50 and 1,100.00 for bicluster_0006 and 240.00 and 390.00 for bicluster_0515 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -65 -0.1 0.749994 CDS
Motif 1 Motif 2 Residual
bicluster_0006
e.value: 
8.5
Motif Bicluster: 
e.value: 
1100
Motif Bicluster: 
0.61
bicluster_0515
e.value: 
240
Motif Bicluster: 
e.value: 
390
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
3-isopropylmalate dehydrogenase 3-isopropylmalate dehydrogenase
Operon # Operon
1956 Rv2995c - Rv2996c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-isopropylmalate dehydrogenase Valine, leucine and isoleucine biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610132 NP_217511.1 Run
GO:0003862

3-isopropylmalate dehydrogenase activity

3-isopropylmalate dehydrogenase activity

Details: 
Catalysis of the reaction: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD+ = 3-carboxy-4-methyl-2-oxopentanoate + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0003862

3-isopropylmalate dehydrogenase activity

3-isopropylmalate dehydrogenase activity

Details: 
Catalysis of the reaction: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD+ = 3-carboxy-4-methyl-2-oxopentanoate + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.