Rv3003c Acetolactate synthase large subunit (EC 2.2.1.6)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3003c ilvB1 Acetolactate synthase large subunit (EC 2.2.1.6) CDS 3361130 3362986 - 1 857 618 FALSE

Rv3003c (Acetolactate synthase large subunit (EC 2.2.1.6)) is predicted to be co-regulated in modules bicluster_0295 with residual 0.48 and bicluster_0462 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 5,200.00 for bicluster_0295 and 2,900.00 and 12,000.00 for bicluster_0462 respectively.

These modules are enriched for following go terms: organonitrogen compound biosynthetic pro..., organonitrogen compound metabolic proces..., NAD biosynthetic process, nicotinamide nucleotide biosynthetic pro..., NAD metabolic process, pyridine nucleotide biosynthetic process, acetolactate synthase activity, transferase activity, transferring aldeh... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Acetolactate synthase acetolactate synthase 1 catalytic subunit
Operon # Operon
1962 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

C5-Branched dibasic acid metabolism

9
Total items in this category:  

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117044 YP_177917.1 Run
GO:0003984

acetolactate synthase activity

acetolactate synthase activity

Details: 
Catalysis of the reaction: 2 pyruvate = 2-acetolactate + CO2.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: