Rv3065 Multidrug resistance protein mmr

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3065 mmr Multidrug resistance protein mmr CDS 3430387 3430710 + 324 107 FALSE

Rv3065 (Multidrug resistance protein mmr) is predicted to be co-regulated in modules bicluster_0385 with residual 0.48 and bicluster_0534 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.63 and 9.20 for bicluster_0385 and 0.00 and 0.02 for bicluster_0534 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18313 MT3150.1 1992
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 10
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator TrcR
No 1 -0.06 0.972379 Primary.TSS
No 67 0.28 0.473448 Primary.TSS
Transcriptional regulator, TetR family
Repressed 1 -2.28 1.44e-22 Primary.TSS
Phosphatidylinositol alpha-mannosyltransferase (EC 2.4.1.57)
NA 7 Primary.TSS
transcriptional regulator, ArsR family
Induced 36 1.64 0.00000000000000152 Primary.TSS
Possible oxidoreductase subunit
No -57 0.17 0.770097 Primary.TSS
Transcriptional regulatory protein
Induced 1 0.75 0.0136544 Primary.TSS
No -118 -0.07 0.955062 Primary.TSS
Transcriptional regulator, TetR family
No -9 0.02 0.982433 Primary.TSS
Transcriptional regulator, AsnC family
No -87 -0.11 0.949904 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0385
e.value: 
0.63
Motif Bicluster: 
e.value: 
9.2
Motif Bicluster: 
0.48
bicluster_0534
e.value: 
0.0000000021
Motif Bicluster: 
e.value: 
0.017
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Multidrug resistance protein mmr multidrugs-transport integral membrane protein MMR
Operon # Operon
2009 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117052 YP_177922.1 Run
GO:0015562

efflux transmembrane transporter activity

efflux transmembrane transporter activity

Details: 
Catalysis of the transfer of a specific substance or related group of substances from the inside of the cell to the outside of the cell across a membrane.
GO Category: 
molecular_function
9
Total items in this category:  
GO:0022857

transmembrane transporter activity

transmembrane transporter activity

Details: 
Enables the transfer of a substance from one side of a membrane to the other.
GO Category: 
molecular_function
4
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.550000 1.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: