Rv3068c Phosphoglucomutase (EC 5.4.2.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3068c pgmA Phosphoglucomutase (EC 5.4.2.2) CDS 3431979 3433622 - 1 644 547 FALSE

Rv3068c (Phosphoglucomutase (EC 5.4.2.2)) is predicted to be co-regulated in modules bicluster_0256 with residual 0.48 and bicluster_0471 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.27 for bicluster_0256 and 0.00 and 0.08 for bicluster_0471 respectively.

These modules are enriched for following go terms: carbohydrate metabolic process, transferase activity, transferring hexos....

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14981 MT3153 399
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
\Transcriptional regulator, XRE family\\\""
Repressed 60 -0.64 0.0297076 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0256
e.value: 
0.014
Motif Bicluster: 
e.value: 
0.27
Motif Bicluster: 
0.48
bicluster_0471
e.value: 
0.00000000026
Motif Bicluster: 
e.value: 
0.079
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHOGLUCOMUTASE PGMA [GLUCOSE PHOSPHOMUTASE] [PGM] phosphoglucomutase
Operon # Operon
2011 Rv3068c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610205 NP_217584.1 Run
GO:0004614

phosphoglucomutase activity

phosphoglucomutase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.420000 18.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.