Rv3068c Phosphoglucomutase (EC 5.4.2.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3068c pgmA Phosphoglucomutase (EC 5.4.2.2) CDS 3431979 3433622 - 1 644 547 FALSE

Rv3068c (Phosphoglucomutase (EC 5.4.2.2)) is predicted to be co-regulated in modules bicluster_0256 with residual 0.48 and bicluster_0471 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.27 for bicluster_0256 and 0.00 and 0.08 for bicluster_0471 respectively.

These modules are enriched for following go terms: carbohydrate metabolic process, transferase activity, transferring hexos....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14981 MT3153 399
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
\Transcriptional regulator, XRE family\\\""
Repressed 60 -0.64 0.0297076 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0256
e.value: 
0.014
Motif Bicluster: 
e.value: 
0.27
Motif Bicluster: 
0.48
bicluster_0471
e.value: 
0.00000000026
Motif Bicluster: 
e.value: 
0.079
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHOGLUCOMUTASE PGMA [GLUCOSE PHOSPHOMUTASE] [PGM] phosphoglucomutase
Operon # Operon
2011
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610205 NP_217584.1 Run
GO:0004614

phosphoglucomutase activity

phosphoglucomutase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.420000 18.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: