Rv3075c Citrate lyase beta chain (EC 4.1.3.6)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3075c Citrate lyase beta chain (EC 4.1.3.6) CDS 3438050 3438973 - 924 307 FALSE

Rv3075c (Citrate lyase beta chain (EC 4.1.3.6)) is predicted to be co-regulated in modules bicluster_0026 with residual 0.58 and bicluster_0500 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.18 and 1.60 for bicluster_0026 and 0.00 and 0.44 for bicluster_0500 respectively.

These modules are enriched for following go terms: phosphopantetheine binding, modified amino acid binding, amide binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.23 47 3439011 3438964 3438973
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2015
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Citrate (pro-3S)-lyase Citrate cycle (TCA cycle)
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610212 NP_217591.1 Run
GO:0008815

citrate (pro-3S)-lyase activity

citrate (pro-3S)-lyase activity

Details: 
Catalysis of the reaction: citrate = acetate + oxaloacetate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.950000 0.92

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: