Rv3086 Alcohol dehydrogenase (EC 1.1.1.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3086 adhD Alcohol dehydrogenase (EC 1.1.1.1) CDS 3451781 3452887 + 1 107 368 FALSE

Rv3086 (Alcohol dehydrogenase (EC 1.1.1.1)) is predicted to be co-regulated in modules bicluster_0245 with residual 0.51 and bicluster_0413 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.15 and 240.00 for bicluster_0245 and 0.12 and 0.15 for bicluster_0413 respectively.

These modules are enriched for following go terms: oxidoreductase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0245
e.value: 
0.15
Motif Bicluster: 
e.value: 
240
Motif Bicluster: 
0.51
bicluster_0413
e.value: 
0.12
Motif Bicluster: 
e.value: 
0.15
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
PROBABLE ZINC-TYPE ALCOHOL DEHYDROGENASE ADHD [ALDEHYDE REDUCTASE] zinc-type alcohol dehydrogenase AdhD
Operon # Operon
2021 - - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Linoleic acid metabolism

3
Total items in this category:  

KEGG

Chloroalkane and chloroalkene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610223 NP_217602.1 Run
GO:0001101

response to acid

response to acid

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an acid stimulus.
GO Category: 
biological_process
11
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.800000 0.99

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: