Rv3097c (MTCY164.08c), len: 436. Esterase/lipase, identical to TR:P77909 (EMBL:U76006) ESTERASE/LIPASE (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) (437 aa); N-terminal similar to N-terminal of M. tuberculosis PE family members, eg Y03A_MYCTU Q10637 hypothetical glycine-rich 49.6 kd pro (603 aa), fasta scores, opt: 334 E(): 4.8e-13, (43.4% identity in 152 aa overlap). Other relatives include MTCY1A11.25c, 7.8e-14; MTCY21B4.13c, 2.4e-12; MTCY270.06, 5.4e-12; MTCY359.33, 1.2e-11; MTC1A11.04, 1.2ee-11; cY130.seq, 1.3e-11

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3097c lipY (MTCY164.08c), len: 436. Esterase/lipase, identical to TR:P77909 (EMBL:U76006) ESTERASE/LIPASE (EC... CDS 3465778 3467091 - 1 314 437 FALSE

Rv3097c ((MTCY164.08c), len: 436. Esterase/lipase, identical to TR:P77909 (EMBL:U76006) ESTERASE/LIPASE (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) (437 aa); N-terminal similar to N-terminal of M. tuberculosis PE family members, eg Y03A_MYCTU Q10637 hypothetical glycine-rich 49.6 kd pro (603 aa), fasta scores, opt: 334 E(): 4.8e-13, (43.4% identity in 152 aa overlap). Other relatives include MTCY1A11.25c, 7.8e-14; MTCY21B4.13c, 2.4e-12; MTCY270.06, 5.4e-12; MTCY359.33, 1.2e-11; MTC1A11.04, 1.2ee-11; cY130.seq, 1.3e-11) is predicted to be co-regulated in modules bicluster_0160 with residual 0.59 and bicluster_0398 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 5,000.00 for bicluster_0160 and 3.30 and 23.00 for bicluster_0398 respectively.

These modules are enriched for following go terms: ligase activity, forming carbon-nitrogen....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PE-PGRS FAMILY PROTEIN PROBABLY TRIACYLGLYCEROL LIPASE [ESTERASE_LIPASE] [TRIGLYCERIDE LIPASE] [TRIBUTYRASE] triacylglycerol lipase
Operon # Operon
2026
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Triacylglycerol lipase Glycerolipid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycerolipid metabolism

11
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117054 YP_177924.1 Run
GO:0004806

triglyceride lipase activity

triglyceride lipase activity

Details: 
Catalysis of the reaction: triacylglycerol + H2O = diacylglycerol + a fatty acid anion.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0016042

lipid catabolic process

lipid catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
1
Total items in this category:  
GO:0042594

response to starvation

response to starvation

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a starvation stimulus, deprivation of nourishment.
GO Category: 
biological_process
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.370000 0.79

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: