Rv3105c Peptide chain release factor 2

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3105c prfB Peptide chain release factor 2 CDS 3472768 3473904 - 1 137 378 FALSE

Rv3105c (Peptide chain release factor 2) is predicted to be co-regulated in modules bicluster_0025 with residual 0.54 and bicluster_0129 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.31 and 8.20 for bicluster_0025 and 0.00 and 2.40 for bicluster_0129 respectively.

These modules are enriched for following go terms: nucleotide-sugar metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-1.463 21 3473883 3473883 3473904
Product (LegacyBRC) Product (RefSeq)
Peptide chain release factor 2 peptide chain release factor 2
Operon # Operon
2029 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610242 NP_217621.1 Run
GO:0006415

translational termination

translational termination

Details: 
The process resulting in the release of a polypeptide chain from the ribosome, usually in response to a termination codon (UAA, UAG, or UGA in the universal genetic code).
GO Category: 
biological_process
3
Total items in this category:  
GO:0003747

translation release factor activity

translation release factor activity

Details: 
Involved in catalyzing the release of a nascent polypeptide chain from a ribosome.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: