Rv3106 Ferredoxin--NADP(+) reductase, actinobacterial (eukaryote-like) type (EC 1.18.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3106 fprA Ferredoxin--NADP(+) reductase, actinobacterial (eukaryote-like) type (EC 1.18.1.2) CDS 3474007 3475377 + 1 371 456 FALSE

Rv3106 (Ferredoxin--NADP(+) reductase, actinobacterial (eukaryote-like) type (EC 1.18.1.2)) is predicted to be co-regulated in modules bicluster_0129 with residual 0.53 and bicluster_0177 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 2.40 for bicluster_0129 and 3.10 and 110.00 for bicluster_0177 respectively.

These modules are enriched for following go terms: glutamine metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15077 MT3189 1319
Product (LegacyBRC) Product (RefSeq)
NADPH-ferredoxin reductase fprA NADPH:adrenodoxin oxidoreductase FPRA (NADPH-ferredoxin reductase)
Operon # Operon
2030
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Ferredoxin--NADP(+) reductase Photosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610243 NP_217622.1 Run
GO:0004324

ferredoxin-NADP+ reductase activity

ferredoxin-NADP+ reductase activity

Details: 
Catalysis of the reaction: reduced ferredoxin + NADP+ = oxidized ferredoxin + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008860

ferredoxin-NAD+ reductase activity

ferredoxin-NAD+ reductase activity

Details: 
Catalysis of the reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
GO:0070401

NADP+ binding

NADP+ binding

Details: 
Interacting selectively and non-covalently with the oxidized form, NADP+, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.750000 1.92

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: