Rv3116 Sulfur carrier protein adenylyltransferase ThiF

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3116 moeB2 Sulfur carrier protein adenylyltransferase ThiF CDS 3482776 3483945 + 1 170 389 FALSE

Rv3116 (Sulfur carrier protein adenylyltransferase ThiF) is predicted to be co-regulated in modules bicluster_0259 with residual 0.51 and bicluster_0551 with residual 0.63.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0259 and 390.00 and 4,300.00 for bicluster_0551 respectively.

These modules are enriched for following go terms: DNA metabolic process, single-organism cellular process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14894 MT3198 185
Product (LegacyBRC) Product (RefSeq)
PROBABLE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOEB2 [MPT-SYNTHASE SULFURYLASE] [MOLYBDOPTERIN SYNTHASE SULPHURYLASE] molybdenum cofactor biosynthesis protein MoeB
Operon # Operon
2037 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Sulfur relay system

18
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117059 YP_177929.1 Run
GO:0003824

catalytic activity

catalytic activity

Details: 
Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO Category: 
molecular_function
12
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.650000 1.24

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: