Rv3121 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3121 cyp141 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) CDS 3486509 3487711 + 1 203 400 FALSE

Rv3121 (Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)) is predicted to be co-regulated in modules bicluster_0003 with residual 0.55 and bicluster_0465 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 120.00 and 14,000.00 for bicluster_0003 and 1,700.00 and 6,700.00 for bicluster_0465 respectively.

These modules are enriched for following go terms: nucleobase-containing compound biosynthe....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.2 3486671 3486509
Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15086 MT3203 1788
Product (LegacyBRC) Product (RefSeq)
Putative cytochrome P450 141 cytochrome P450 141
Operon # Operon
2038
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610258 NP_217637.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.840000 1.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: