Rv3130c Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3130c tgs1 Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase CDS 3494975 3496366 - 1 392 463 FALSE

Rv3130c (Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase) is predicted to be co-regulated in modules bicluster_0044 with residual 0.54 and bicluster_0182 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.50 and 3.40 for bicluster_0044 and 0.00 and 0.32 for bicluster_0182 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15104 MT3216 2147
Product (LegacyBRC) Product (RefSeq)
Rv3130c triacylglycerol synthase
Operon # Operon
2046 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610266 NP_217646.1 Run
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0004144

diacylglycerol O-acyltransferase activity

diacylglycerol O-acyltransferase activity

Details: 
Catalysis of the reaction: acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0019432

triglyceride biosynthetic process

triglyceride biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a triglyceride, any triester of glycerol.
GO Category: 
biological_process
1
Total items in this category:  
GO:0045017

glycerolipid biosynthetic process

glycerolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycerolipids, any lipid with a glycerol backbone.
GO Category: 
biological_process
7
Total items in this category:  
GO:0047196

long-chain-alcohol O-fatty-acyltransferase activity

long-chain-alcohol O-fatty-acyltransferase activity

Details: 
Catalysis of the reaction: a long-chain-alcohol + acyl-CoA = a long-chain ester + CoA.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0071731

response to nitric oxide

response to nitric oxide

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nitric oxide stimulus.
GO Category: 
biological_process
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.830000 3.64

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: