Rv3140 Probable acyl-CoA dehydrogenase (EC 1.3.99.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3140 fadE23 Probable acyl-CoA dehydrogenase (EC 1.3.99.3) CDS 3506790 3507995 + 1 206 401 FALSE

Rv3140 (Probable acyl-CoA dehydrogenase (EC 1.3.99.3)) is predicted to be co-regulated in modules bicluster_0156 with residual 0.52 and bicluster_0593 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 180.00 and 890.00 for bicluster_0156 and 4,900.00 and 7,400.00 for bicluster_0593 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
No 9 -0.18 0.999734 CDS
WhiB family transcriptional regulator
No 19 0.05 0.938847 CDS
Arsenical resistance operon repressor
No -4 -0.35 0.586101 CDS
transcriptional regulator, ArsR family
Induced 3 1.09 0.000000535 CDS
Motif 1 Motif 2 Residual
bicluster_0156
e.value: 
180
Motif Bicluster: 
e.value: 
890
Motif Bicluster: 
0.52
bicluster_0593
e.value: 
4900
Motif Bicluster: 
e.value: 
7400
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-CoA DEHYDROGENASE FADE23 acyl-CoA dehydrogenase FADE23
Operon # Operon
2052 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610276 NP_217656.1 Run
GO:0003995

acyl-CoA dehydrogenase activity

acyl-CoA dehydrogenase activity

Details: 
Catalysis of the reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.
GO Category: 
molecular_function
8
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.040000 0.30

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: