Rv3167c Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3167c Transcriptional regulator, TetR family CDS 3535431 3536057 - 627 208 TRUE

Rv3167c (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0127 with residual 0.45 and bicluster_0453 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.02 for bicluster_0127 and 60.00 and 620.00 for bicluster_0453 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 5 of 5
Gene Target Differential Expression Distance Expression pvalue Type
No -117 0.16 0.779232 Primary.TSS
Cell division protein DivIC (FtsB), stabilizes FtsL against RasP cleavage
No -37 0.11 0.812352 CDS
Type VII secretion integral membrane protein EccD
No -65 0.08 0.698558 Primary.TSS
Threonine dehydrogenase and related Zn-dependent dehydrogenases
Repressed -104 -1.79 0.00000000000189 CDS
Molybdopterin biosynthesis protein MoeW
No -53 0.42 0.53829 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN [PROBABLY TETR-FAMILY] TetR family transcriptional regulator
Operon # Operon
2062
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610303 NP_217683.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426936 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426937 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426938 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv3167c_B272 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.420000 1.08

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.0179321
p-value INH: 0.860973
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 11.40 U
D3I 3 3 3.83 11.03 I
D3U 3 3 3.83 11.47 U
D5I 9 5 6.00 8.34 I
D5U 17 5 6.00 8.65 U
D7I 18 7 8.14 10.35 I
D7U 19 7 8.14 11.21 U
D14I 4 14 15.63 8.64 I
D14U 4 14 15.63 11.65 U
D17I 3 17 19.15 6.43 I
D17U 3 17 19.15 11.54 U
D21I 4 21 23.23 5.79 I
D21U 4 21 23.23 11.65 U
D24I 3 24 26.60 2.29 I
D24U 3 24 26.60 11.34 U
D28I 4 28 30.61 4.75 I
D28U 4 28 30.61 11.38 U