Rv3170 Amine oxidase [flavin-containing] A (EC 1.4.3.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3170 aofH Amine oxidase [flavin-containing] A (EC 1.4.3.4) CDS 3538505 3539851 + 1 347 448 FALSE

Rv3170 (Amine oxidase [flavin-containing] A (EC 1.4.3.4)) is predicted to be co-regulated in modules bicluster_0338 with residual 0.49 and bicluster_0538 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,500.00 and 3,800.00 for bicluster_0338 and 21.00 and 880.00 for bicluster_0538 respectively.

These modules are enriched for following go terms: alpha,alpha-trehalose-phosphate synthase..., UDP-glucosyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0338
e.value: 
3500
Motif Bicluster: 
e.value: 
3800
Motif Bicluster: 
0.49
bicluster_0538
e.value: 
21
Motif Bicluster: 
e.value: 
880
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
Putative flavin-containing monoamine oxidase aofH flavin-containing monoamine oxidase
Operon # Operon
2064
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Phenylalanine metabolism

13
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610306 NP_217686.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.310000 0.44

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: