Rv3199c NADH pyrophosphatase (EC 3.6.1.22)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3199c nudC NADH pyrophosphatase (EC 3.6.1.22) CDS 3571602 3572543 - 942 313 FALSE

Rv3199c (NADH pyrophosphatase (EC 3.6.1.22)) is predicted to be co-regulated in modules bicluster_0201 with residual 0.51 and bicluster_0217 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 78.00 and 500.00 for bicluster_0201 and 0.00 and 0.09 for bicluster_0217 respectively.

These modules are enriched for following go terms: vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce..., cofactor metabolic process, carboxylic acid biosynthetic process, organic acid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0201
e.value: 
78
Motif Bicluster: 
e.value: 
500
Motif Bicluster: 
0.51
bicluster_0217
e.value: 
0.00032
Motif Bicluster: 
e.value: 
0.091
Motif Bicluster: 
0.47
Product (LegacyBRC) Product (RefSeq)
NADH pyrophosphatase NADH pyrophosphatase
Operon # Operon
2089
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NAD(+) diphosphatase Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610335 NP_217715.1 Run
GO:0000210

NAD+ diphosphatase activity

NAD+ diphosphatase activity

Details: 
Catalysis of the reaction: NAD+ + H2O = AMP + NMN.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 0.38

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: