Rv3206c Sulfur carrier protein adenylyltransferase ThiF

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3206c moeB1 Sulfur carrier protein adenylyltransferase ThiF CDS 3582532 3583710 - 1 179 392 FALSE

Rv3206c (Sulfur carrier protein adenylyltransferase ThiF) is predicted to be co-regulated in modules bicluster_0106 with residual 0.48 and bicluster_0425 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.82 and 3.10 for bicluster_0106 and 4,100.00 and 3,500.00 for bicluster_0425 respectively.

These modules are enriched for following go terms: oxo-acid-lyase activity DNA ligase activity, ligase activity, forming phosphoric este....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOEB1 [MPT-SYNTHASE SULFURYLASE] [MOLYBDOPTERIN SYNTHASE SULPHURYLASE] molybdopterin biosynthesis-like protein MoeZ
Operon # Operon
2093 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Sulfur relay system

18
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117072 YP_177942.1 Run
GO:0003824

catalytic activity

catalytic activity

Details: 
Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0008146

sulfotransferase activity

sulfotransferase activity

Details: 
Catalysis of the transfer of a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate to the hydroxyl group of an acceptor, producing the sulfated derivative and 3'-phosphoadenosine 5'-phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0019344

cysteine biosynthetic process

cysteine biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of cysteine, 2-amino-3-mercaptopropanoic acid.
GO Category: 
biological_process
4
Total items in this category:  
GO:0042783

active evasion of host immune response

active evasion of host immune response

Details: 
Any mechanism of immune avoidance that directly affects the host immune system, e.g. blocking any stage in host MHC class I and II presentation. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
20
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: