Rv3221c Biotin carboxyl carrier protein

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3221c TB7.3 Biotin carboxyl carrier protein CDS 3597551 3597766 - 216 71 FALSE

Rv3221c (Biotin carboxyl carrier protein) is predicted to be co-regulated in modules bicluster_0382 with residual 0.57 and bicluster_0458 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.26 for bicluster_0382 and 0.15 and 30,000.00 for bicluster_0458 respectively.

These modules are enriched for following go terms: organonitrogen compound metabolic proces..., primary metabolic process, small molecule metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, organonitrogen compound biosynthetic pro....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv3221c putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
Operon # Operon
2108 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610357 NP_217737.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: