Rv3220c Signal transduction histidine kinase, subgroup 2

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3220c Signal transduction histidine kinase, subgroup 2 CDS 3596029 3597534 - 1 506 501 FALSE

Rv3220c (Signal transduction histidine kinase, subgroup 2) is predicted to be co-regulated in modules bicluster_0012 with residual 0.58 and bicluster_0382 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 0.11 for bicluster_0012 and 0.00 and 0.26 for bicluster_0382 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18851 MT3316 3167
Product (LegacyBRC) Product (RefSeq)
PROBABLE TWO COMPONENT SENSOR KINASE two component sensor kinase
Operon # Operon
2106 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610356 NP_217736.1 Run
GO:0000155

two-component sensor activity

two-component sensor activity

Details: 
Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0000160

two-component signal transduction system (phosphorelay)

two-component signal transduction system (phosphorelay)

Details: 
A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
GO Category: 
biological_process
4
Total items in this category:  
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 1.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: