Rv3247c Thymidylate kinase (EC 2.7.4.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3247c tmk Thymidylate kinase (EC 2.7.4.9) CDS 3627419 3628063 - 645 214 FALSE

Rv3247c (Thymidylate kinase (EC 2.7.4.9)) is predicted to be co-regulated in modules bicluster_0098 with residual 0.64 and bicluster_0128 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0098 and 0.00 and 0.00 for bicluster_0128 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0098
e.value: 
0
Motif Bicluster: 
e.value: 
3.3e-26
Motif Bicluster: 
0.64
bicluster_0128
e.value: 
0
Motif Bicluster: 
e.value: 
3.6e-35
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Thymidylate kinase thymidylate kinase
Operon # Operon
2126
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

dTMP kinase Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610383 NP_217764.1 Run
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004798

thymidylate kinase activity

thymidylate kinase activity

Details: 
Catalysis of the reaction: ATP + thymidine 5'-phosphate = ADP + thymidine 5'-diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0046044

TMP metabolic process

TMP metabolic process

Details: 
The chemical reactions and pathways involving TMP, ribosylthymine monophosphate.
GO Category: 
biological_process
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: