Rv3252c Alkane-1 monooxygenase (EC 1.14.15.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3252c alkB Alkane-1 monooxygenase (EC 1.14.15.3) CDS 3630738 3631988 - 1 251 416 FALSE

Rv3252c (Alkane-1 monooxygenase (EC 1.14.15.3)) is predicted to be co-regulated in modules bicluster_0063 with residual 0.56 and bicluster_0167 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 37.00 for bicluster_0063 and 0.00 and 49.00 for bicluster_0167 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17939 MT3350 953
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSMEMBRANE ALKANE 1-MONOOXYGENASE ALKB [ALKANE 1-HYDROXYLASE] [LAURIC ACID OMEGA-HYDROXYLASE] [OMEGA-HYDROXYLASE] [FATTY ACID OMEGA-HYDROXYLASE] [ALKANE HYDROXYLASE-RUBREDOXIN] transmembrane alkane 1-monooxygenase AlkB
Operon # Operon
2128 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610388 NP_217769.1 Run
GO:0018685

alkane 1-monooxygenase activity

alkane 1-monooxygenase activity

Details: 
Catalysis of the reaction: octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0080133

midchain alkane hydroxylase activity

midchain alkane hydroxylase activity

Details: 
Catalysis of the conversion of an alkane to a secondary alcohol.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.020000 0.77

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: