Rv3252c Alkane-1 monooxygenase (EC 1.14.15.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3252c alkB Alkane-1 monooxygenase (EC 1.14.15.3) CDS 3630738 3631988 - 1 251 416 FALSE

Rv3252c (Alkane-1 monooxygenase (EC 1.14.15.3)) is predicted to be co-regulated in modules bicluster_0063 with residual 0.56 and bicluster_0167 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 37.00 for bicluster_0063 and 0.00 and 49.00 for bicluster_0167 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17939 MT3350 953
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 7 of 7
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, PadR family
No 29 0.29 0.962221 Primary.TSS
Histone protein Lsr2
No 27 0.3 0.561244 Primary.TSS
Possible oxidoreductase subunit
No 27 -0.45 0.234748 Primary.TSS
DNA-binding response regulator TrcR
No 7 0.46 0.399115 Primary.TSS
HTH-type transcriptional regulator
No 34 -0.25 0.540912 Primary.TSS
putative two-component system response regulator
No 34 -0.02 0.97281 Primary.TSS
Transcriptional regulatory protein
Induced -2 1.22 0.00000871 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0063
e.value: 
0.014
Motif Bicluster: 
e.value: 
37
Motif Bicluster: 
0.56
bicluster_0167
e.value: 
0.0021
Motif Bicluster: 
e.value: 
49
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSMEMBRANE ALKANE 1-MONOOXYGENASE ALKB [ALKANE 1-HYDROXYLASE] [LAURIC ACID OMEGA-HYDROXYLASE] [OMEGA-HYDROXYLASE] [FATTY ACID OMEGA-HYDROXYLASE] [ALKANE HYDROXYLASE-RUBREDOXIN] transmembrane alkane 1-monooxygenase AlkB
Operon # Operon
2128 Rv3249c - Rv3250c - Rv3251c - Rv3252c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610388 NP_217769.1 Run
GO:0018685

alkane 1-monooxygenase activity

alkane 1-monooxygenase activity

Details: 
Catalysis of the reaction: octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0080133

midchain alkane hydroxylase activity

midchain alkane hydroxylase activity

Details: 
Catalysis of the conversion of an alkane to a secondary alcohol.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.020000 0.77

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.