Rv3257c Phosphomannomutase (EC 5.4.2.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3257c manB Phosphomannomutase (EC 5.4.2.8) CDS 3637312 3638709 - 1 398 465 FALSE

Rv3257c (Phosphomannomutase (EC 5.4.2.8)) is predicted to be co-regulated in modules bicluster_0100 with residual 0.55 and bicluster_0405 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 310.00 for bicluster_0100 and 0.58 and 2.90 for bicluster_0405 respectively.

These modules are enriched for following go terms: oxidoreductase activity, oxidoreductase activity, acting on NAD(P... alcohol metabolic process, phospholipid metabolic process, phospholipid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0100
e.value: 
130
Motif Bicluster: 
e.value: 
310
Motif Bicluster: 
0.55
bicluster_0405
e.value: 
0.58
Motif Bicluster: 
e.value: 
2.9
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHOMANNOMUTASE PMMA [PMM] [PHOSPHOMANNOSE MUTASE] phosphomannomutase/phosphoglucomutase
Operon # Operon
2131 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610393 NP_217774.1 Run
GO:0004615

phosphomannomutase activity

phosphomannomutase activity

Details: 
Catalysis of the reaction: alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004614

phosphoglucomutase activity

phosphoglucomutase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004615

phosphomannomutase activity

phosphomannomutase activity

Details: 
Catalysis of the reaction: alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: