Rv3257c Phosphomannomutase (EC 5.4.2.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3257c manB Phosphomannomutase (EC 5.4.2.8) CDS 3637312 3638709 - 1 398 465 FALSE

Rv3257c (Phosphomannomutase (EC 5.4.2.8)) is predicted to be co-regulated in modules bicluster_0100 with residual 0.55 and bicluster_0405 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 310.00 for bicluster_0100 and 0.58 and 2.90 for bicluster_0405 respectively.

These modules are enriched for following go terms: oxidoreductase activity, oxidoreductase activity, acting on NAD(P... alcohol metabolic process, phospholipid metabolic process, phospholipid biosynthetic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0100
e.value: 
130
Motif Bicluster: 
e.value: 
310
Motif Bicluster: 
0.55
bicluster_0405
e.value: 
0.58
Motif Bicluster: 
e.value: 
2.9
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHOMANNOMUTASE PMMA [PMM] [PHOSPHOMANNOSE MUTASE] phosphomannomutase/phosphoglucomutase
Operon # Operon
2131 Rv3255c - Rv3256c - Rv3257c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610393 NP_217774.1 Run
GO:0004615

phosphomannomutase activity

phosphomannomutase activity

Details: 
Catalysis of the reaction: alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004614

phosphoglucomutase activity

phosphoglucomutase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004615

phosphomannomutase activity

phosphomannomutase activity

Details: 
Catalysis of the reaction: alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.