Rv3290c Probable L-lysine-epsilon aminotransferase (EC 2.6.1.36) (L-lysine aminotransferase) (Lysine 6-aminotransferase)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3290c lat Probable L-lysine-epsilon aminotransferase (EC 2.6.1.36) (L-lysine aminotransferase) (Lysine 6-... CDS 3670445 3671794 - 1 350 449 FALSE

Rv3290c (Probable L-lysine-epsilon aminotransferase (EC 2.6.1.36) (L-lysine aminotransferase) (Lysine 6-aminotransferase)) is predicted to be co-regulated in modules bicluster_0300 with residual 0.49 and bicluster_0560 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.40 for bicluster_0300 and 0.01 and 21.00 for bicluster_0560 respectively.

These modules are enriched for following go terms: tricarboxylic acid cycle enzyme complex, acid-thiol ligase activity, ligase activity, forming carbon-sulfur b... folic acid-containing compound biosynthe..., pteridine-containing compound biosynthet..., coenzyme metabolic process, cellular modified amino acid biosyntheti....

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 13
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
Repressed 44 -0.82 0.000209577 Primary.TSS
Transcriptional repressor EthR, TetR family
No 42 -0.1 0.955181 Primary.TSS
HTH-type transcriptional regulator
No 52 0.05 0.92429 Primary.TSS
Transcriptional regulatory protein
Induced 60 1.92 0.0000000000655 Primary.TSS
Histone protein Lsr2
No 46 -0.08 0.918974 Primary.TSS
Phosphatidylinositol alpha-mannosyltransferase (EC 2.4.1.57)
NA 37 Primary.TSS
Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system
Induced 44 2.69 4.51e-18 Primary.TSS
putative two-component system response regulator
No 48 0.25 0.644091 Primary.TSS
TetR/ACRR family transcriptional regulator
No -107 0.29 0.962042 Primary.TSS
DNA-binding response regulator TrcR
No 25 0.8 0.0702245 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0300
e.value: 
0.00000045
Motif Bicluster: 
e.value: 
1.4
Motif Bicluster: 
0.49
bicluster_0560
e.value: 
0.014
Motif Bicluster: 
e.value: 
21
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
Probable L-lysine-epsilon aminotransferase L-lysine aminotransferase
Operon # Operon
2150 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

L-lysine 6-transaminase Lysine biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Lysine biosynthesis

15
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610426 NP_217807.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.370000 1.49

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: