Rv3295 Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3295 Transcriptional regulator, TetR family CDS 3676066 3676731 + 666 221 FALSE

Rv3295 (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0268 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 51.00 and 620.00 for bicluster_0268 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process phosphorylation, cellular respiration, energy derivation by oxidation of organi....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:12
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14787 MT3394 324
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN [PROBABLY TETR-FAMILY] TetR family transcriptional regulator
Operon # Operon
2154
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610431 NP_217812.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.12

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00000000199904
p-value INH: 0.863117
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 15.10 U
D3I 3 3 3.83 15.09 I
D3U 3 3 3.83 14.64 U
D5I 9 5 6.00 13.36 I
D5U 17 5 6.00 13.66 U
D7I 18 7 8.14 14.10 I
D7U 19 7 8.14 15.30 U
D14I 4 14 15.63 12.01 I
D14U 4 14 15.63 15.48 U
D17I 3 17 19.15 8.66 I
D17U 3 17 19.15 15.55 U
D21I 4 21 23.23 8.40 I
D21U 4 21 23.23 15.51 U
D24I 3 24 26.60 5.87 I
D24U 3 24 26.60 15.46 U
D28I 4 28 30.61 7.05 I
D28U 4 28 30.61 15.14 U