Rv3303c Dihydrolipoamide dehydrogenase (EC 1.8.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3303c lpdA Dihydrolipoamide dehydrogenase (EC 1.8.1.4) CDS 3689457 3690938 - 1 482 493 FALSE

Rv3303c (Dihydrolipoamide dehydrogenase (EC 1.8.1.4)) is predicted to be co-regulated in modules bicluster_0164 with residual 0.60 and bicluster_0522 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0164 and 0.00 and 0.00 for bicluster_0522 respectively.

These modules are enriched for following go terms: cation transmembrane transporter activit....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE DIHYDROLIPOAMIDE DEHYDROGENASE LPDA [LIPOAMIDE REDUCTASE [NADH]] [LIPOYL DEHYDROGENASE] [DIHYDROLIPOYL DEHYDROGENASE] [DIAPHORASE] flavoprotein disulfide reductase
Operon # Operon
2157 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610439 NP_217820.1 Run
GO:0004148

dihydrolipoyl dehydrogenase activity

dihydrolipoyl dehydrogenase activity

Details: 
Catalysis of the reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0003955

NAD(P)H dehydrogenase (quinone) activity

NAD(P)H dehydrogenase (quinone) activity

Details: 
Catalysis of the reaction: NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0016655

oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor

oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which NADH or NADPH acts as a hydrogen or electron donor and reduces a quinone or a similar acceptor molecule.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
GO:0070401

NADP+ binding

NADP+ binding

Details: 
Interacting selectively and non-covalently with the oxidized form, NADP+, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0016668

oxidoreductase activity, acting on a sulfur group of donors, NAD or NADP as acceptor

oxidoreductase activity, acting on a sulfur group of donors, NAD or NADP as acceptor

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which a sulfur-containing group acts as a hydrogen or electron donor and reduces NAD or NADP.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.830000 0.70

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: