Rv3317 Succinate dehydrogenase hydrophobic membrane anchor protein

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3317 sdhD Succinate dehydrogenase hydrophobic membrane anchor protein CDS 3704437 3704871 + 435 144 FALSE

Rv3317 (Succinate dehydrogenase hydrophobic membrane anchor protein) is predicted to be co-regulated in modules bicluster_0186 with residual 0.45 and bicluster_0469 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.18 for bicluster_0186 and 0.00 and 0.00 for bicluster_0469 respectively.

These modules are enriched for following go terms: generation of precursor metabolites and ..., oxidation-reduction process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE SUCCINATE DEHYDROGENASE [HYDROPHOBIC MEMBRANE ANCHOR SUBUNIT] SDHD [SUCCINIC DEHYDROGENASE] [FUMARATE REDUCTASE] [FUMARATE DEHYDROGENASE] [FUMARIC HYDROGENASE] succinate dehydrogenase hydrophobic membrane anchor subunit SdhD
Operon # Operon
2166 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610453 NP_217834.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.280000 0.26

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: