Rv3319 Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3319 sdhB Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) CDS 3706772 3707563 + 792 263 FALSE

Rv3319 (Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1)) is predicted to be co-regulated in modules bicluster_0186 with residual 0.45 and bicluster_0469 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.18 for bicluster_0186 and 0.00 and 0.00 for bicluster_0469 respectively.

These modules are enriched for following go terms: generation of precursor metabolites and ..., oxidation-reduction process .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0186
e.value: 
0.00035
Motif Bicluster: 
e.value: 
0.18
Motif Bicluster: 
0.45
bicluster_0469
e.value: 
6.1e-25
Motif Bicluster: 
e.value: 
0.0000000000000038
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
PROBABLE SUCCINATE DEHYDROGENASE [IRON-SULPHUR PROTEIN SUBUNIT] SDHB [SUCCINIC DEHYDROGENASE] [FUMARATE REDUCTASE] [FUMARATE DEHYDROGENASE] [FUMARIC HYDROGENASE] succinate dehydrogenase iron-sulfur subunit
Operon # Operon
2167 Rv3318 - Rv3319
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610455 NP_217836.1 Run
GO:0000104

succinate dehydrogenase activity

succinate dehydrogenase activity

Details: 
Catalysis of the reaction: succinate + acceptor = fumarate + reduced acceptor.
GO Category: 
molecular_function
7
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.430000 0.92

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.