Rv3345c COG0536: GTP-binding protein Obg

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3345c PE_PGRS50 COG0536: GTP-binding protein Obg CDS 3738158 3742774 - 4 617 1 538 FALSE

Rv3345c (COG0536: GTP-binding protein Obg) is predicted to be co-regulated in modules bicluster_0343 with residual 0.46 and bicluster_0542 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 390.00 and 13,000.00 for bicluster_0343 and 0.02 and 150.00 for bicluster_0542 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.176 3742768 3742774
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0343
e.value: 
390
Motif Bicluster: 
e.value: 
13000
Motif Bicluster: 
0.46
bicluster_0542
e.value: 
0.019
Motif Bicluster: 
e.value: 
150
Motif Bicluster: 
0.60
Product (LegacyBRC) Product (RefSeq)
PE-PGRS FAMILY PROTEIN PE-PGRS family protein
Operon # Operon
2184 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Arylsulfatase C21-Steroid hormone metabolism, Sphingolipid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117094 YP_177962.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.300000 1.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: