Rv3370c DNA polymerase III alpha subunit (EC 2.7.7.7)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3370c dnaE2 DNA polymerase III alpha subunit (EC 2.7.7.7) CDS 3781501 3784776 - 3 276 1 091 FALSE

Rv3370c (DNA polymerase III alpha subunit (EC 2.7.7.7)) is predicted to be co-regulated in modules bicluster_0027 with residual 0.56 and bicluster_0539 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.04 for bicluster_0027 and 0.00 and 0.98 for bicluster_0539 respectively.

These modules are enriched for following go terms: disaccharide metabolic process, trehalose metabolic process, trehalose biosynthetic process, disaccharide biosynthetic process, carbohydrate biosynthetic process NADH dehydrogenase (ubiquinone) activity, NADH dehydrogenase (quinone) activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
\Transcriptional regulator, XRE family\\\""
No 58 -0.39 0.0778838 CDS
Transcriptional regulator kstR (Rv3574), TetR family
No 34 -0.19 0.40338 CDS
transcriptional regulator, ArsR family
No 44 -0.29 0.0542259 CDS
Motif 1 Motif 2 Residual
bicluster_0027
e.value: 
0.0000000000000019
Motif Bicluster: 
e.value: 
0.036
Motif Bicluster: 
0.56
bicluster_0539
e.value: 
0.000001
Motif Bicluster: 
e.value: 
0.98
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
Error-prone DNA polymerase error-prone DNA polymerase
Operon # Operon
2203
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

DNA-directed DNA polymerase Pyrimidine metabolism, Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
161352461 NP_217887.2 Run
GO:0003887

DNA-directed DNA polymerase activity

DNA-directed DNA polymerase activity

Details: 
Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and primer.
GO Category: 
molecular_function
8
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0046677

response to antibiotic

response to antibiotic

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
GO Category: 
biological_process
15
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.520000 1.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: