Rv3372 Trehalose-6-phosphate phosphatase (EC 3.1.3.12)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3372 otsB2 Trehalose-6-phosphate phosphatase (EC 3.1.3.12) CDS 3786314 3787489 + 1 176 391 FALSE

Rv3372 (Trehalose-6-phosphate phosphatase (EC 3.1.3.12)) is predicted to be co-regulated in modules bicluster_0027 with residual 0.56 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.04 for bicluster_0027 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: disaccharide metabolic process, trehalose metabolic process, trehalose biosynthetic process, disaccharide biosynthetic process, carbohydrate biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0027
e.value: 
0.0000000000000019
Motif Bicluster: 
e.value: 
0.036
Motif Bicluster: 
0.56
bicluster_0490
e.value: 
0.0085
Motif Bicluster: 
e.value: 
730
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TREHALOSE 6-PHOSPHATE PHOSPHATASE OTSB2 [TREHALOSE-PHOSPHATASE] [TPP] trehalose 6-phosphate phosphatase
Operon # Operon
2205
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Trehalose-phosphatase Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610508 NP_217889.1 Run
GO:0004805

trehalose-phosphatase activity

trehalose-phosphatase activity

Details: 
Catalysis of the reaction: trehalose 6-phosphate + H2O = trehalose + phosphate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004805

trehalose-phosphatase activity

trehalose-phosphatase activity

Details: 
Catalysis of the reaction: trehalose 6-phosphate + H2O = trehalose + phosphate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: