Rv3376 Hydrolase, haloacid dehalogenase-like family

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3376 Hydrolase, haloacid dehalogenase-like family CDS 3790156 3790809 + 654 217 FALSE

Rv3376 (Hydrolase, haloacid dehalogenase-like family) is predicted to be co-regulated in modules bicluster_0251 with residual 0.46 and bicluster_0566 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 780.00 and 7,200.00 for bicluster_0251 and 130.00 and 3,800.00 for bicluster_0566 respectively.

These modules are enriched for following go terms: cysteine metabolic process, L-serine metabolic process, serine family amino acid biosynthetic pr..., sulfur amino acid biosynthetic process, small molecule biosynthetic process, single-organism biosynthetic process, sulfur amino acid metabolic process, alpha-amino acid biosynthetic process DNA recombination, transposase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2207
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610512 NP_217893.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.780000 2.83

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: