Rv3379c 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3379c dxs2 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7) CDS 3793257 3794867 - 1 611 536 FALSE

Rv3379c (1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)) is predicted to be co-regulated in modules bicluster_0114 with residual 0.42 and bicluster_0597 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 150.00 and 7,000.00 for bicluster_0114 and 0.00 and 0.28 for bicluster_0597 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18300 MT3489 1963
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0114
e.value: 
150
Motif Bicluster: 
e.value: 
7000
Motif Bicluster: 
0.42
bicluster_0597
e.value: 
0.0034
Motif Bicluster: 
e.value: 
0.28
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
PROBABLE 1-DEOXY-D-XYLULOSE 5-PHOSPHATE SYNTHASE DXS2 [1-DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE] [DXP SYNTHASE] [DXPS] 1-deoxy-D-xylulose-5-phosphate synthase
Operon # Operon
2208 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

1-deoxy-D-xylulose-5-phosphate synthase Terpenoid backbone biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Terpenoid backbone biosynthesis

27
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610515 NP_217896.1 Run
GO:0008661

1-deoxy-D-xylulose-5-phosphate synthase activity

1-deoxy-D-xylulose-5-phosphate synthase activity

Details: 
Catalysis of the reaction: D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO(2).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.56

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: