Rv3393 Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3393 iunH Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) CDS 3808461 3809387 + 927 308 FALSE

Rv3393 (Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)) is predicted to be co-regulated in modules bicluster_0070 with residual 0.60 and bicluster_0287 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.21 for bicluster_0070 and 0.00 and 4.30 for bicluster_0287 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0070
e.value: 
0.012
Motif Bicluster: 
e.value: 
0.21
Motif Bicluster: 
0.60
bicluster_0287
e.value: 
0.0046
Motif Bicluster: 
e.value: 
4.3
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
PROBABLE NUCLEOSIDE HYDROLASE IUNH [PURINE NUCLEOSIDASE] nucleoside hydrolase IunH
Operon # Operon
2219
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Purine nucleosidase Purine metabolism, Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610529 NP_217910.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.130000 0.73

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: