Rv3396c GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3396c guaA GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2) CDS 3812501 3814078 - 1 578 525 FALSE

Rv3396c (GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)) is predicted to be co-regulated in modules bicluster_0209 with residual 0.57 and bicluster_0251 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1,300.00 for bicluster_0209 and 780.00 and 7,200.00 for bicluster_0251 respectively.

These modules are enriched for following go terms: cysteine metabolic process, L-serine metabolic process, serine family amino acid biosynthetic pr..., sulfur amino acid biosynthetic process, small molecule biosynthetic process, single-organism biosynthetic process, sulfur amino acid metabolic process, alpha-amino acid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv3396c GMP synthase
Operon # Operon
2223 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610532 NP_217913.1 Run
GO:0003922

GMP synthase (glutamine-hydrolyzing) activity

GMP synthase (glutamine-hydrolyzing) activity

Details: 
Catalysis of the reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: