Rv3409c Cholesterol oxidase (EC 1.1.3.6)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3409c choD Cholesterol oxidase (EC 1.1.3.6) CDS 3826991 3828727 - 1 737 578 FALSE

Rv3409c (Cholesterol oxidase (EC 1.1.3.6)) is predicted to be co-regulated in modules bicluster_0019 with residual 0.52 and bicluster_0080 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 27.00 and 3,700.00 for bicluster_0019 and 0.00 and 0.00 for bicluster_0080 respectively.

These modules are enriched for following go terms: fatty acid biosynthetic process, monocarboxylic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15741 MT3517 1944
Product (LegacyBRC) Product (RefSeq)
PROBABLE CHOLESTEROL OXIDASE CHOD [CHOLESTEROL-O2 OXIDOREDUCTASE] cholesterol oxidase precursor
Operon # Operon
2230
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cholesterol oxidase Steroid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Steroid biosynthesis

2
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610545 NP_217926.1 Run
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.470000 1.35

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: