Rv3433c NAD(P)HX epimerase / NAD(P)HX dehydratase

Product Feature Type Start End Strand Length AA Length is TF
Rv3433c NAD(P)HX epimerase / NAD(P)HX dehydratase CDS 3851792 3853213 - 1 422 473 FALSE

Rv3433c (NAD(P)HX epimerase / NAD(P)HX dehydratase) is predicted to be co-regulated in modules bicluster_0169 with residual 0.34 and bicluster_0178 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 60.00 and 2,800.00 for bicluster_0169 and 140.00 and 36,000.00 for bicluster_0178 respectively.

These modules are enriched for following go terms: aminoglycan biosynthetic process, glycosaminoglycan biosynthetic process, peptidoglycan biosynthetic process, cell wall macromolecule biosynthetic pro..., cellular component macromolecule biosynt..., cell wall macromolecule metabolic proces..., peptidoglycan metabolic process, aminoglycan metabolic process, peptidoglycan-based cell wall biogenesis, glycosaminoglycan metabolic process, cell wall biogenesis, cell wall organization or biogenesis .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv3433c_MT3539
Operon # Operon
2246 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610569 NP_217950.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.680000 4.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: