Rv3436c Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3436c glmS Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) CDS 3855015 3856889 - 1 875 624 FALSE

Rv3436c (Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)) is predicted to be co-regulated in modules bicluster_0052 with residual 0.48 and bicluster_0301 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 460.00 and 2,100.00 for bicluster_0052 and 0.00 and 4.40 for bicluster_0301 respectively.

These modules are enriched for following go terms: divalent metal ion transport, divalent inorganic cation transport, transition metal ion transport, divalent inorganic cation transmembrane ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, IclR family
No -73 -0.24 0.63314 Internal.TSS
Transcriptional regulator, MarR family
Repressed 69 -1.71 1.23e-17 CDS
Motif 1 Motif 2 Residual
bicluster_0052
e.value: 
460
Motif Bicluster: 
e.value: 
2100
Motif Bicluster: 
0.48
bicluster_0301
e.value: 
0.00091
Motif Bicluster: 
e.value: 
4.4
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] glucosamine--fructose-6-phosphate aminotransferase
Operon # Operon
2247
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610572 NP_217953.1 Run
GO:0004360

glutamine-fructose-6-phosphate transaminase (isomerizing) activity

glutamine-fructose-6-phosphate transaminase (isomerizing) activity

Details: 
Catalysis of the reaction: beta-D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: