Rv3436c Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3436c glmS Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) CDS 3855015 3856889 - 1 875 624 FALSE

Rv3436c (Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)) is predicted to be co-regulated in modules bicluster_0052 with residual 0.48 and bicluster_0301 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 460.00 and 2,100.00 for bicluster_0052 and 0.00 and 4.40 for bicluster_0301 respectively.

These modules are enriched for following go terms: divalent metal ion transport, divalent inorganic cation transport, transition metal ion transport, divalent inorganic cation transmembrane ....

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, IclR family
No -73 -0.24 0.63314 Internal.TSS
Transcriptional regulator, MarR family
Repressed 69 -1.71 1.23e-17 CDS
Motif 1 Motif 2 Residual
bicluster_0052
e.value: 
460
Motif Bicluster: 
e.value: 
2100
Motif Bicluster: 
0.48
bicluster_0301
e.value: 
0.00091
Motif Bicluster: 
e.value: 
4.4
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] glucosamine--fructose-6-phosphate aminotransferase
Operon # Operon
2247 Rv3436c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610572 NP_217953.1 Run
GO:0004360

glutamine-fructose-6-phosphate transaminase (isomerizing) activity

glutamine-fructose-6-phosphate transaminase (isomerizing) activity

Details: 
Catalysis of the reaction: beta-D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.