Rv3468c UDP-glucose 4-epimerase (EC 5.1.3.2)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3468c UDP-glucose 4-epimerase (EC 5.1.3.2) CDS 3884975 3886069 - 1 095 364 FALSE

Rv3468c (UDP-glucose 4-epimerase (EC 5.1.3.2)) is predicted to be co-regulated in modules bicluster_0039 with residual 0.54 and bicluster_0404 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 290.00 for bicluster_0039 and 230.00 and 97.00 for bicluster_0404 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18733 MT3574 2576
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
WhiB family transcriptional regulator
Induced 65 0.6 0.00350286 CDS
Motif 1 Motif 2 Residual
bicluster_0039
e.value: 
0.0013
Motif Bicluster: 
e.value: 
290
Motif Bicluster: 
0.54
bicluster_0404
e.value: 
230
Motif Bicluster: 
e.value: 
97
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
POSSIBLE DTDP-GLUCOSE 46-DEHYDRATASE dTDP-glucose 4,6-dehydratase
Operon # Operon
2266 Rv3468c - Rv3469c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117109 YP_177974.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.010000 0.68

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.