Rv3516 Enoyl-CoA hydratase (EC 4.2.1.17)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3516 echA19 Enoyl-CoA hydratase (EC 4.2.1.17) CDS 3952544 3953335 + 792 263 FALSE

Rv3516 (Enoyl-CoA hydratase (EC 4.2.1.17)) is predicted to be co-regulated in modules bicluster_0200 with residual 0.45 and bicluster_0528 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.16 for bicluster_0200 and 0.00 and 47.00 for bicluster_0528 respectively.

These modules are enriched for following go terms: coenzyme binding, oxidoreductase activity .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.979 27 3952499 3952526 3952544
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator kstR (Rv3574), TetR family
Repressed -35 -1.07 0.00000795 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0200
e.value: 
8.3e-28
Motif Bicluster: 
e.value: 
0.16
Motif Bicluster: 
0.45
bicluster_0528
e.value: 
0.00023
Motif Bicluster: 
e.value: 
47
Motif Bicluster: 
0.42
Product (LegacyBRC) Product (RefSeq)
POSSIBLE ENOYL-CoA HYDRATASE ECHA19 [ENOYL HYDRASE] [UNSATURATED ACYL-CoA HYDRATASE] [CROTONASE] enoyl-CoA hydratase
Operon # Operon
2296 Rv3516
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610652 NP_218033.1 Run
GO:0004300

enoyl-CoA hydratase activity

enoyl-CoA hydratase activity

Details: 
Catalysis of the reaction: (3S)-3-hydroxyacyl-CoA = trans-2-enoyl-CoA + H2O.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.090000 0.62

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.