Rv3516 Enoyl-CoA hydratase (EC 4.2.1.17)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3516 echA19 Enoyl-CoA hydratase (EC 4.2.1.17) CDS 3952544 3953335 + 792 263 FALSE

Rv3516 (Enoyl-CoA hydratase (EC 4.2.1.17)) is predicted to be co-regulated in modules bicluster_0200 with residual 0.45 and bicluster_0528 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.16 for bicluster_0200 and 0.00 and 47.00 for bicluster_0528 respectively.

These modules are enriched for following go terms: coenzyme binding, oxidoreductase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.979 27 3952499 3952526 3952544
Product (LegacyBRC) Product (RefSeq)
POSSIBLE ENOYL-CoA HYDRATASE ECHA19 [ENOYL HYDRASE] [UNSATURATED ACYL-CoA HYDRATASE] [CROTONASE] enoyl-CoA hydratase
Operon # Operon
2296
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610652 NP_218033.1 Run
GO:0004300

enoyl-CoA hydratase activity

enoyl-CoA hydratase activity

Details: 
Catalysis of the reaction: (3S)-3-hydroxyacyl-CoA = trans-2-enoyl-CoA + H2O.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.090000 0.62

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: