Rv3523 3-ketoacyl-CoA thiolase

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3523 ltp3 3-ketoacyl-CoA thiolase CDS 3959529 3960713 + 1 185 394 FALSE

Rv3523 (3-ketoacyl-CoA thiolase) is predicted to be co-regulated in modules bicluster_0058 with residual 0.53 and bicluster_0249 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.10 for bicluster_0058 and 0.00 and 0.00 for bicluster_0249 respectively.

These modules are enriched for following go terms: acetyl-CoA C-acyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18009 MT3623 1120
Product (LegacyBRC) Product (RefSeq)
PROBABLE LIPID CARRIER PROTEIN OR KETO ACYL-COA THIOLASE LTP3 acetyl-CoA acetyltransferase
Operon # Operon
2301 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Synthesis and degradation of ketone bodies

12
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610659 NP_218040.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.870000 1.83

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: