Rv3546 Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3546 fadA5 Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9) CDS 3985557 3986732 + 1 176 391 FALSE

Rv3546 (Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9)) is predicted to be co-regulated in modules bicluster_0166 with residual 0.50 and bicluster_0200 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0166 and 0.00 and 0.16 for bicluster_0200 respectively.

These modules are enriched for following go terms: coenzyme binding, oxidoreductase activity.

This gene is found to be essential for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Two component transcriptional regulatory protein DevR
Induced -72 1.17 0.00000000167 Primary.TSS
Transcriptional regulator, TetR family
No -80 0.66 0.582795 Primary.TSS
Transcriptional regulator, ArsR family
No -66 -0.34 0.180127 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0166
e.value: 
0.0000016
Motif Bicluster: 
e.value: 
0.004
Motif Bicluster: 
0.50
bicluster_0200
e.value: 
8.3e-28
Motif Bicluster: 
e.value: 
0.16
Motif Bicluster: 
0.45
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACETYL-CoA ACETYLTRANSFERASE FADA5 [ACETOACETYL-CoA THIOLASE] acetyl-CoA acetyltransferase
Operon # Operon
2313 Rv3546
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Synthesis and degradation of ketone bodies

12
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610682 NP_218063.1 Run
GO:0003988

acetyl-CoA C-acyltransferase activity

acetyl-CoA C-acyltransferase activity

Details: 
Catalysis of the reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
essential 0.000000 0.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.