Rv3546 Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3546 fadA5 Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9) CDS 3985557 3986732 + 1 176 391 FALSE

Rv3546 (Probable acetyl-CoA acetyltransferase FadA5 (EC 2.3.1.9)) is predicted to be co-regulated in modules bicluster_0166 with residual 0.50 and bicluster_0200 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0166 and 0.00 and 0.16 for bicluster_0200 respectively.

These modules are enriched for following go terms: coenzyme binding, oxidoreductase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Two component transcriptional regulatory protein DevR
Induced -72 1.17 0.00000000167 Primary.TSS
Transcriptional regulator, TetR family
No -80 0.66 0.582795 Primary.TSS
Transcriptional regulator, ArsR family
No -66 -0.34 0.180127 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0166
e.value: 
0.0000016
Motif Bicluster: 
e.value: 
0.004
Motif Bicluster: 
0.50
bicluster_0200
e.value: 
8.3e-28
Motif Bicluster: 
e.value: 
0.16
Motif Bicluster: 
0.45
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACETYL-CoA ACETYLTRANSFERASE FADA5 [ACETOACETYL-CoA THIOLASE] acetyl-CoA acetyltransferase
Operon # Operon
2313
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Synthesis and degradation of ketone bodies

12
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610682 NP_218063.1 Run
GO:0003988

acetyl-CoA C-acyltransferase activity

acetyl-CoA C-acyltransferase activity

Details: 
Catalysis of the reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: