Rv3551 Putative CoA-transferase subunit alpha Rv3551/MT3655 (EC 2.8.3.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3551 Putative CoA-transferase subunit alpha Rv3551/MT3655 (EC 2.8.3.-) CDS 3989896 3990774 + 879 292 FALSE

Rv3551 (Putative CoA-transferase subunit alpha Rv3551/MT3655 (EC 2.8.3.-)) is predicted to be co-regulated in modules bicluster_0152 with residual 0.50 and bicluster_0181 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.09 for bicluster_0152 and 0.00 and 88.00 for bicluster_0181 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on the C..., flavin adenine dinucleotide binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0152
e.value: 
0.00000000028
Motif Bicluster: 
e.value: 
0.094
Motif Bicluster: 
0.50
bicluster_0181
e.value: 
0.000000000000033
Motif Bicluster: 
e.value: 
88
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Putative CoA-transferase subunit alpha Rv3551_MT3655 CoA-transferase subunit alpha
Operon # Operon
2316 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610687 NP_218068.1 Run
GO:0044117

growth of symbiont in host

growth of symbiont in host

Details: 
The increase in size or mass of an organism, occurring within the cells or tissues of the host organism. This may (but not necessarily) include a filamentous growth form, and also can include secretion of proteases and lipases to break down host tissue. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
36
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: