Rv3554 Possible electron transfer protein FdxB (EC 1.-.-.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3554 fdxB Possible electron transfer protein FdxB (EC 1.-.-.-) CDS 3992685 3994742 + 2 058 685 FALSE

Rv3554 (Possible electron transfer protein FdxB (EC 1.-.-.-)) is predicted to be co-regulated in modules bicluster_0181 with residual 0.51 and bicluster_0504 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 88.00 for bicluster_0181 and 0.00 and 3.10 for bicluster_0504 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on the C..., flavin adenine dinucleotide binding nitrogen cycle metabolic process, transition metal ion binding, hydrolase activity, acting on carbon-nit..., metal ion binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.792 3992724 3992685
Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18371 MT3658 2119
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
No -27 0.3 0.259177 CDS
Transcriptional regulator, TetR family
Induced 3 2.37 3.25e-21 CDS
Motif 1 Motif 2 Residual
bicluster_0181
e.value: 
0.000000000000033
Motif Bicluster: 
e.value: 
88
Motif Bicluster: 
0.51
bicluster_0504
e.value: 
0.0025
Motif Bicluster: 
e.value: 
3.1
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
POSSIBLE ELECTRON TRANSFER PROTEIN FDXB electron transfer protein FdxB
Operon # Operon
2317 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610690 NP_218071.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.950000 1.85

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: