Rv3580c Cysteinyl-tRNA synthetase (EC 6.1.1.16)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3580c cysS1 Cysteinyl-tRNA synthetase (EC 6.1.1.16) CDS 4022394 4023803 - 1 410 469 FALSE

Rv3580c (Cysteinyl-tRNA synthetase (EC 6.1.1.16)) is predicted to be co-regulated in modules bicluster_0128 with residual 0.56 and bicluster_0504 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0128 and 0.00 and 3.10 for bicluster_0504 respectively.

These modules are enriched for following go terms: nitrogen cycle metabolic process, transition metal ion binding, hydrolase activity, acting on carbon-nit..., metal ion binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Cysteinyl-tRNA synthetase 1 cysteinyl-tRNA synthetase
Operon # Operon
2332 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine--tRNA ligase Aminoacyl-tRNA biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Aminoacyl-tRNA biosynthesis

69
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117128 YP_177992.1 Run
GO:0004817

cysteine-tRNA ligase activity

cysteine-tRNA ligase activity

Details: 
Catalysis of the reaction: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: