Rv3585 DNA repair protein RadA

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3585 radA DNA repair protein RadA CDS 4026444 4027886 + 1 443 480 FALSE

Rv3585 (DNA repair protein RadA) is predicted to be co-regulated in modules bicluster_0230 with residual 0.33 and bicluster_0250 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,600.00 and 2,200.00 for bicluster_0230 and 91.00 and 3,000.00 for bicluster_0250 respectively.

These modules are enriched for following go terms: single-organism process, nucleic acid metabolic process, hydrolase activity, endodeoxyribonuclease activity, serine-type endopeptidase activity, nucleoside-triphosphatase activity, pyrophosphatase activity, hydrolase activity, acting on acid anhyd..., hydrolase activity, acting on acid anhyd..., deoxyribonuclease activity, ATPase activity, coupled, DNA-dependent ATPase activity, DNA helicase activity, serine-type peptidase activity, serine hydrolase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
DNA repair protein radA homolog DNA repair protein RadA
Operon # Operon
2335 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610721 NP_218102.1 Run
GO:0003678

DNA helicase activity

DNA helicase activity

Details: 
Catalysis of the reaction: NTP + H2O = NDP + phosphate, to drive the unwinding of a DNA helix.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0006260

DNA replication

DNA replication

Details: 
The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
GO Category: 
biological_process
2
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: