Rv3602c Pantoate--beta-alanine ligase (EC 6.3.2.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3602c panC Pantoate--beta-alanine ligase (EC 6.3.2.1) CDS 4044281 4045210 - 930 309 FALSE

Rv3602c (Pantoate--beta-alanine ligase (EC 6.3.2.1)) is predicted to be co-regulated in modules bicluster_0370 with residual 0.49 and bicluster_0556 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 38.00 for bicluster_0370 and 0.02 and 1.40 for bicluster_0556 respectively.

These modules are enriched for following go terms: cobalamin binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, AraC family
No 8 0.29 0.904758 CDS
Motif 1 Motif 2 Residual
bicluster_0370
e.value: 
0.024
Motif Bicluster: 
e.value: 
38
Motif Bicluster: 
0.49
bicluster_0556
e.value: 
0.023
Motif Bicluster: 
e.value: 
1.4
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
Pantothenate synthetase pantoate--beta-alanine ligase
Operon # Operon
2346 - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610738 NP_218119.1 Run
GO:0004592

pantoate-beta-alanine ligase activity

pantoate-beta-alanine ligase activity

Details: 
Catalysis of the reaction: ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004592

pantoate-beta-alanine ligase activity

pantoate-beta-alanine ligase activity

Details: 
Catalysis of the reaction: ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0015940

pantothenate biosynthetic process

pantothenate biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of pantothenate, the anion of pantothenic acid. It is a B complex vitamin that is a constituent of coenzyme A and is distributed ubiquitously in foods.
GO Category: 
biological_process
2
Total items in this category:  
GO:0019482

beta-alanine metabolic process

beta-alanine metabolic process

Details: 
The chemical reactions and pathways involving beta-alanine (3-aminopropanoic acid), an achiral amino acid and an isomer of alanine. It occurs free (e.g. in brain) and in combination (e.g. in pantothenate) but it is not a constituent of proteins.
GO Category: 
biological_process
1
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: