Rv3606c 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3606c folK 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) CDS 4048181 4048747 - 567 188 FALSE

Rv3606c (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3)) is predicted to be co-regulated in modules bicluster_0135 with residual 0.54 and bicluster_0562 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 2,200.00 for bicluster_0135 and 1,500.00 and 6,400.00 for bicluster_0562 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, aromatic compound biosynthetic process, heterocycle biosynthetic process, organic cyclic compound biosynthetic pro... .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -123 -0.2 0.999609 CDS
Transcriptional regulator, PadR family
No -107 0.2 0.529906 CDS
Transcriptional regulator, PadR family
No 60 0.2 0.529906 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0135
e.value: 
3.1
Motif Bicluster: 
e.value: 
2200
Motif Bicluster: 
0.54
bicluster_0562
e.value: 
1500
Motif Bicluster: 
e.value: 
6400
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridi ne pyrophosphokinase FolK
Operon # Operon
2348 Rv3605c - Rv3606c - Rv3607c - Rv3608c - Rv3609c - Rv3610c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Folate biosynthesis

10
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610742 NP_218123.1 Run
GO:0003848

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity

Details: 
Catalysis of the reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + ATP = (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + AMP + 2 H(+).
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.