Rv3645 Adenylate cyclase (EC 4.6.1.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3645 Adenylate cyclase (EC 4.6.1.1) CDS 4082807 4084456 + 1 650 549 FALSE

Rv3645 (Adenylate cyclase (EC 4.6.1.1)) is predicted to be co-regulated in modules bicluster_0411 with residual 0.41 and bicluster_0593 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 36.00 for bicluster_0411 and 4,900.00 and 7,400.00 for bicluster_0593 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.215 60 4082867 4082867 4082807
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 12
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -126 -0.43 0.0458589 Internal.TSS
Transcriptional regulatory protein
No -23 0.23 0.184032 CDS
Transcriptional regulator, TetR family
No -66 -0.43 0.0458589 CDS
Transcriptional regulator, TetR family
No -87 0.01 0.992554 Antisense.TSS
DNA-binding response regulator mtrA
No -94 -0.09 0.881671 Internal.TSS
Transcriptional regulator, TetR family
No -74 -0.08 0.815465 CDS
Transcriptional regulator, AsnC family
No -83 -0.12 0.900955 Internal.TSS
Transcriptional regulator, TetR family
No 68 0.01 0.992554 Internal.TSS
Transcriptional regulatory protein
No -83 0.23 0.184032 Internal.TSS
Transcriptional regulator, TetR family
No -134 -0.08 0.815465 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0411
e.value: 
0.016
Motif Bicluster: 
e.value: 
36
Motif Bicluster: 
0.41
bicluster_0593
e.value: 
4900
Motif Bicluster: 
e.value: 
7400
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED TRANSMEMBRANE PROTEIN transmembrane protein
Operon # Operon
2373
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Adenylate cyclase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610781 NP_218162.1 Run
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: