Rv3657c FIG054221: Possible conserved alanine rich membrane protein

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3657c FIG054221: Possible conserved alanine rich membrane protein CDS 4095540 4096115 - 576 191 FALSE

Rv3657c (FIG054221: Possible conserved alanine rich membrane protein) is predicted to be co-regulated in modules bicluster_0064 with residual 0.44 and bicluster_0185 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 500.00 and 2,500.00 for bicluster_0064 and 1,500.00 and 29,000.00 for bicluster_0185 respectively.

These modules are enriched for following go terms: protein secretion, secretion by cell, secretion, establishment of localization in cell, cellular localization, antibiotic metabolic process, drug metabolic process, protein transport, establishment of protein localization .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.604 4096142 4096115
Product (LegacyBRC) Product (RefSeq)
POSSIBLE CONSERVED ALANINE RICH MEMBRANE PROTEIN
Operon # Operon
2381 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610793 NP_218174.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.79

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: